LAUSR

We investigated the effect of apigenin, a dietary flavonoid, on isoproterenol hydrochloride (ISO)‐induced apoptotic signaling in cardiomyoblast H9C2 cells. The results showed that apigenin treatment (10 µM) prevented ISO (31.25 μM)‐induced lipid peroxidative levels and antioxidants status in H9C2 cells. Furthermore, apigenin inhibited expression of inflammatory markers in ISO‐treated cells. In addition, apigenin prevented ISO‐induced DNA damage and apoptotic signaling through modulating the expression of Bax, caspase‐3, ‐8 and ‐9, cytochrome c, and Fas proteins in H9C2 cells. It is concluded that apigenin prevents ISO‐induced antioxidants depletion, oxidative DNA damage, inflammatory, and apoptotic signaling in H9C2 cells. Thus, the present results demonstrated that apigenin has a cardioprotective effect on cardiomyoblasts cells.