LAUSR

This study aims to investigate the combined effects of Pulsed wave low‐level laser therapy (PW LLLT) and human bone marrow mesenchymal stem cell‐conditioned medium (hBM‐MSC‐CM) on the microbial flora and tensiometrical properties of an infected wound model with methicillin‐resistant staphylococcal aureus (MRSA) in an experimental model for Type 1 diabetes mellitus (TIDM). TIDM was induced in rats by streptozotocin (STZ). One full‐thickness excision was made on the backs of the rats. Next, the rats were divided into the following groups: Group 1 was the control (placebo) group; Group 2 received hBM‐MSCs‐CM four times; Group 3 were laser PWLLLT (890 nm, 80 Hz, 0.2 J/cm2); and Group 4 received hBM‐MSCs‐CM +LASER. Wounds were infected with MRSA. Microbiological examinations were performed on days 4, 7, and 15. Tensiometerical examinations were carried out on the 15th day. One‐way analysis of variance showed that laser and CM alone and/or in combination significantly increases the tensiomerical properties of the repaired wounds compared with control wounds. A combination of PW laser and CM was statistically more effective than other treated groups. Two‐way analysis of variance showed that laser and CM alone and/or in combination significantly decreases the colony‐forming units (CFUs) compared with the control group. The application of hBM‐MSC‐CM and PWlaser alone and/or together significantly accelerates the wound‐healing process in MRSA‐infected cutaneous wounds in TI DM in rats. Additionally, a combined application of hBM‐MSC‐CM and PWlaser demonstrates a synergistic effect on the wound‐healing process in MRSA‐infected cutaneous wounds in Type I DM rats.