LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Human gingival derived neuronal cells in the optimized caffeic acid hydrogel for hemitransection spinal cord injury model

Photo from wikipedia

Spinal cord injury induces scar formation causes axonal damage that leads to the degeneration of axonal function. Still, there is no robust conceptual design to regenerate the damaged axon after… Click to show full abstract

Spinal cord injury induces scar formation causes axonal damage that leads to the degeneration of axonal function. Still, there is no robust conceptual design to regenerate the damaged axon after spinal injury. Therefore, the present study demonstrates that human gingival derived neuronal stem cells (GNSCs) transplants in the injectable caffeic acid bioconjugated hydrogel (CBGH) helps to bridge the cavity and promote the engraftment and repopulation of transplants in the injured spinal tissue. Our study reports that the bioluminescence imaging in vivo imaging system (IVIS) provides a satisfactory progression in CBGH‐GNSCs transplants compare to lesion control and CBGH alone. Immune regulators interleukin‐6 (IL‐6), tumor necrosis factor‐α, neutrophil elastase are decreased, IL‐10 is increased. Likewise, immunostaining (TAU/TUJ‐1, SOX‐2/NeuN, MAP‐2/PSD93, NSE, S100b, and GFAP) shown repopulated cells. Also, TRA‐1‐81 expression confirms the absence of immune rejection in the CBGH‐GNSCs transplants. However, locomotor recovery test, gene (IL‐6, CASPASE3, p14‐ARF, VEGF, LCAM, BDNF, NT3, NGN2, TrKc, FGF2, Sox‐2, TUJ‐1, MAP‐2, Nestin, and NeuN) and protein expression (TAU, TUJ‐1, SOX‐2 MAP‐2, PSD93, NeuN, TRA‐1‐81, GFAP, TAU, and MBP) shows functional improvements in the CBGH‐GNSCs group. Further, GABA and glutamine level demonstrates the new synaptic vesicle formation. Hence, the CBGH scaffold enhances GNSCs transplants to restore the injured spinal tissue.

Keywords: gingival derived; spinal cord; derived neuronal; injury; cord injury; human gingival

Journal Title: Journal of Cellular Biochemistry
Year Published: 2019

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.