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Detecting the polymorphism of TERF1 gene by an improved PCR‐RFLP method

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Polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) is a common and mature method of detecting the single nucleotide polymorphism (SNP). But, for the polymorphism site rs3863242 of telomeric repeat binding… Click to show full abstract

Polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) is a common and mature method of detecting the single nucleotide polymorphism (SNP). But, for the polymorphism site rs3863242 of telomeric repeat binding factor 1(TERF1) gene, there is no appropriate restriction enzyme to recognize it, which limits the research between the variants of rs3863242 and human diseases.

Keywords: pcr rflp; terf1 gene; method; polymorphism

Journal Title: Journal of Clinical Laboratory Analysis
Year Published: 2018

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