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Evaluation of LAMP assay using phenotypic tests and PCR for detection of blaKPC gene among clinical samples

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Carbapenem‐resistant Enterobacteriaceae (CRE) infection constitutes a public health threat, which blaKPC was the major carbapenemases concerned in China. Timely and efficient diagnosis is of paramount importance for controlling the spread… Click to show full abstract

Carbapenem‐resistant Enterobacteriaceae (CRE) infection constitutes a public health threat, which blaKPC was the major carbapenemases concerned in China. Timely and efficient diagnosis is of paramount importance for controlling the spread of drug‐resistant bacteria. Here, we develop an approach based on loop‐mediated isothermal amplification (LAMP) for rapid confirmation of blaKPC within 60 min from samples collected.

Keywords: tests pcr; assay using; using phenotypic; lamp assay; evaluation lamp; phenotypic tests

Journal Title: Journal of Clinical Laboratory Analysis
Year Published: 2022

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