LAUSR

miRNAs, a kind of noncoding small RNA, play a significant role in adipose differentiation. In this study, we explored the effect of miR‐324‐5p in adipose differentiation, and found that miR‐324‐5p could promote adipocytes differentiation and increase body weight in mice. We overexpressed miR‐324‐5p during adipocytes differentiation, by oil red O and bodipy staining found that lipid accumulation was increased, and the expression level of adipogenic related genes were significantly increased. And the opposite experimental results were obtained after inhibiting miR‐324‐5p. In vivo, we injected miR‐324‐5p agomiR in obese mice and found that body weight, adipocyte area, and adipogenic‐related gene expression level were significantly increased but lipolytic genes were decreased. To further explore the mechanism of miR‐324‐5p regulation in lipid accumulation, we constructed Krueppel‐like factor 3 (KLF3) 3′‐untranslated region luciferase reporter vector and KLF3 pcDNA 3.1 overexpression vector, and found that miR‐324‐5p was able to directly target KLF3. Overall, in this study we found that miR‐324‐5p could promote mice preadipoytes differentiation and increase mice fat accumulation by targeting KLF3.