LAUSR

Cytoplasmic serine/threonine Pim kinases have emerged as important modulators of immune regulation and oncology. However, their regulatory roles in bone remodeling remain obscure. Here, we aimed to determine the roles of Pim kinases in periodontal disease (PD), focusing on the regulation of osteoclastogenesis and bone resorptive activity. We investigated Pim kinases expression in PD by analyzing data from the online Gene Expression Omnibus database and using ligature‐induced periodontitis mouse model. The expression of Pim kinases during receptor activator of nuclear factor kB ligand (RANKL)‐induced osteoclastogenesis was assessed in mouse bone marrow‐derived macrophages (BMMs) using reverse transcription polymerase chain reaction. Osteoclast differentiation and bone resorption activity were respectively verified by tartrate‐resistant acid phosphatase staining and dentin disc‐based bone resorption assays. We silenced and overexpressed Pim‐2 using small interfering RNA (siRNA) and retroviral vector, respectively, to investigate the molecular mechanisms underlying Pim‐2 regulation in RANKL‐induced osteoclastogenesis and bone resorption activity. Upregulated expression of Pim‐2 was observed in both patients with PD and periodontitis‐affected mouse gingival tissues. siRNA‐mediated silencing of Pim‐2 in BMMs diminished RANKL‐induced resorptive activity without affecting osteoclastogenesis. Moreover, RANKL‐triggered stimulation of a3 isoform, which is a subunit of vacuolar‐type ATPase, was selectively attenuated in BMMs on silencing Pim‐2. The overexpression of Pim‐2 with a retroviral vector stimulated the a3 subunit, thus inducing bone resorption activity. Taken together, these results suggest that Pim‐2 acts as a major modulator of osteoclastic activity by regulating a3 isoform expression in PD.