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Direct genotyping of adenovirus or enterovirus from clinical material using polymerase chain reaction (PCR) followed by Sanger sequencing is often difficult due to the presence of multiple virus types in… Click to show full abstract
Direct genotyping of adenovirus or enterovirus from clinical material using polymerase chain reaction (PCR) followed by Sanger sequencing is often difficult due to the presence of multiple virus types in a sample, or due to varying efficacy of PCR amplifying the capsid gene on the background of foreign nucleic acids. Here we present a simple protocol for virus genotyping using massive parallel amplicon sequencing.
Keywords:
parallel amplicon;
massive parallel;
amplicon sequencing;
adenovirus enterovirus;
virus;
virus genotyping
Journal Title: Journal of Medical Virology
Year Published: 2019
Link to full text (if available)
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Journal Title: Journal of Medical Virology
Year Published: 2019
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!