LAUSR

Anyplex II HPV-28 (HPV-28) can detect individually 28 HPV genotypes. We assessed the agreement between Linear array HPV genotyping (LA-HPV) and HPV-28 for detection of 27 HPV genotypes in 410 stored anogenital samples (75 anal samples, 335 physician-collected cervical samples) collected over 5 years from 410 individuals (13 men, 397 women), including 202 HIV-seropositive individuals. HPV DNA was detected in 393 (95.9%, 95% confidence interval (CI) 93.4-97.4) and 382 (93.2%, 95% CI 90.3-95.3) samples with HPV-28 and LA-HPV (p=0.13), respectively, for a good agreement of 96.3% (kappa=0.65). Of the 10,503 HPV typing results, 10,195 (780 positive, 9,577 negative) were concordant (agreement of 97.1%, 95% CI 96.7-97.4 (kappa=0.82, 95% CI 0.80-0.84). The mean type-specific concordance for 27 genotypes was 97.0%, 95% CI 95.8-98.5 (kappa = 0.86+0.07, 95% CI 0.83-0.88). Excellent agreement was obtained individually for all high-risk genotypes (kappa=0.81-0.97) and for most other genotypes except for types 42, 44, 54, 68 and 69. The mean number of types per sample in discordant samples detected with LA-HPV (3.0, 95% CI 2.7-3.4) was greater than in concordant samples (1.4, 95% CI 1.3-1.5; p<0.001). In conclusion, HPV-28 compared favorably with LA-HPV but was more frequently positive for HPV42 and 68. This article is protected by copyright. All rights reserved.