LAUSR

BACKGROUND This study evaluated the anti-hyperglycemic, anti-bone-resorptive, and anti-inflammatory efficacy of the probiotic Lactobacillus rhamnosus EM1107 in an experimental model of ligature-induced periodontitis in diabetic rats treated with metformin. METHODS 114 male Wistar rats randomly divided into six groups: 1) Control, 2) Experimental Periodontitis (EP), 3) EP + diabetes mellitus (DM), 4) EP + Probiotic (Prob), 5) EP + DM + Prob and 6) EP + DM + Prob + metformin (Met). The animals received probiotic gavage during the 30 days of the experiment. DM was induced on the 14th day of the experiment with a single injection of Streptozotocin (STZ) into the penile vein, followed by ligature for EP induction and metformin gavage on the 19th day, and euthanasia on the 30th day. Heart blood, gingival and periodontal tissue, and hemimaxillae were collected. Biomolecular analysis, immunoenzymatic assays, histomorphology, and microtomographic analysis were performed. Data were statistically analyzed (p<0.05). RESULTS There was a significant reduction in IL-1β and TNF-α in the probiotic groups (p<0.05), as well as in blood glucose levels in the Prob and metformin groups (p<0.001). In addition, the histomorphological analysis revealed that probiotic groups had a reduction in inflammatory infiltrate. TRAP and microtomographic analyzes showed that the EP/DM/Prob/Met group had significantly lower linear and volumetric bone loss than those who had no treatment (p<0.01). SOD and GPx immunostaining decreased in all groups receiving probiotics. CONCLUSION The findings suggest the immuno-inflammatory efficacy of the probiotic Lactobacillus rhamnosus EM1107 administered either alone or in association with metformin in type 1 diabetes mellitus associated with periodontitis. This article is protected by copyright. All rights reserved.