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BACKGROUND This study evaluated the impact of cooking on the profile of bioactive compounds in unripe breadfruit. To this end, the accessibility of bioactive compounds by various solvents was assessed through total phenolic content (TPC) and antioxidant capacity analyses. The most efficient solvent was applied to extract the metabolites that were evaluated by ultra-performance liquid chromatography coupled with high-resolution quadrupole time-of-flight mass spectrometry in MSE mode (UPLC-QTOF-MSE ). RESULTS Cooked and raw breadfruit presented similar TPC and antioxidant capacities in almost all extracts and pure water proved to be the best extractor. Globally, 146 bioactive compounds have been identified for both raw and cooked fruits aqueous extracts. Most of these compounds were stable to the heat treatment applied (121 °C/ 10 minutes). However, results revealed that 22 metabolites contributed to significantly distinguishing the raw from the cooked samples. Among those, 15 compounds such as pyrogallol, 1-acetoxypinoresinol, and scopolin evidenced higher relative abundance in the cooked extracts. On the other hand, only seven metabolites, such as 4-hydroxyhippuric acid, epicatechin, and leptodactylone decreased post-heating. CONCLUSIONS Cooking promoted little alteration in the bioactive compounds profile of immature breadfruit and thus appears to be an exploitation alternative for this perishable fruit, which seems to be a source of a large range of bioactive compounds. This article is protected by copyright. All rights reserved.