LAUSR

BACKGROUND Soluble starch synthase IIa (ALK, SSII-3) is the major gene regulating gelatinization temperature (GT) and SSII-3M1 is an effective marker for identifying SSIIa alleles. However, the haplotypes of SSIIa alleles amplified by SSII-3M1, their allelic effects under different Waxy (Wx) in non-glutinous rice remain unclear. RESULTS Through integrating the genetic background analysis and the identification of genotype of Wx and SSIIa, We found SSIIa alleles amplified by SSII-3 M1 were haplotype 1 (G/G/GC, indica-type) and 4 (A/G/TT, japonica-type), which had significant effect at pasting temperature (PaT), hot paste viscosity (HPV) and alkali spreading value (ASV). There was a significant effects of SSIIa alleles on HPV, cool paste viscosity (CPV) and consistency value under different Wx. Apparent amylose content (AAC) of samples significantly affected the accuracy for GT being represented by Manually- determined pasting temperate (PTm). CONCLUSIONS SSIIa alleles amplified by SSII-3 M1 are indica-type and japonica-type. Different SSIIa haplotypes significantly affect HPV, CPV, PaT and ASV. GT, PaT and PTm are mainly affected by SSIIa alleles. The classification of all samples with different haplotypes of SSIIa indicates that it is essentially according to their AAC (Wx genotypes). Effects of SSIIa alleles are affected by different Wx alleles. This article is protected by copyright. All rights reserved.