LAUSR

BACKGROUND Alcoholic liver disease (ALD) refers to liver damage caused by long-term heavy drinking, which causing oxidative stress and changes in gut microbiota. In this paper, we investigated the hepatoprotective effect of sea buckthorn fermentation liquid on ALD in mice and the interaction between ALD and gut microbiota using animal experiments and gut microbiota measurements. RESULTS We found that the contents of total flavonoids, total triterpenes and related SCFAs in SFL were significantly greater. Liver index, kidney index, spleen index, serum indexes of liver injury (ALT, AST), inflammatory factors in liver tissues (TNF-α, IL-6), oxidation indexes (MDA, SOD) and lipid metabolism indexes (HDL-C LDL-C, TG) suggested that SFL significantly ameliorates liver injury caused by alcohol. By measuring gut microbiota in mice feces samples, we found that the high-dose group of SFL reversed the declining trend of the gut microbiota F/B ratio caused by alcohol; reducing the number of gram-negative bacteroidetes and Patescibacteria was tightly connected with the indicators of ALD. At the genus level, high-dose SFL significantly downregulated Akkermansia, Turicibacter, Alistipes and Ruminiclostridium, and improving the abundance of beneficial bacteria in Lactobacillus. In addition, Alistipes and Ruminiclostridium had a tightly connection with the indicators of ALD. CONCLUSION Sea buckthorn fermentation liquid protected against alcoholic liver disease and modulated the composition of gut microbiota. This article is protected by copyright. All rights reserved.