LAUSR

BACKGROUND The aim of current study was evaluated the synergistic effects of tea polyphenol-ozonated slurry ice (TOSI) on the quality, physicochemical and protein characteristics of large yellow croaker (Pseudosciaena crocea) during chilled (4°C) storage. 0.3% tea polyphenol combined with ozone water was added sodium chloride until the salt concentration reached 3.3% and made the mixture to form the tea polyphenol-ozonated slurry ice (TOSI) by ice machine. Microbial (total viable count (TVC)), physicochemical (total volatile basic nitrogen (TVB-N), K value), myofibrillar fragmentation index (MFI), Ca2+ -ATPase activity, total sulfhydryl content, Intrinsic fluorescence intensity (IFI), Fourier transform infrared (FTIR), scanning electron microscope (SEM) and SDS-PAGE were analyzed during chilled (4°C) storage for up to 20 days. RESULTS The results showed that TOSI could effectively inhibit the increase of TVC and TVB-N, reduce the degree of adenosine triphosphate (ATP) degradation. In addition, the TOSI treatment could protect the integrity of myosin in myofibrillar proteins (MPs) by inhibiting the decrease of Ca2+ -ATPase activity and the content of total sulfhydryl. Furthermore, the TOSI presented a superiorly protective effect on protein structure in MPs as manifested by the results of IFI, FTIR and SDS-PAGE. It was possible that due to the addition of tea polyphenol, which significantly improved the antioxidant activity of this complex. CONCLUSION The TOSI treatment can maintain the quality of large yellow croaker by decreasing the damage of myofibrillar protein caused by the interaction between microorganisms and endogenous enzymes. This article is protected by copyright. All rights reserved.