LAUSR

BACKGROUND Sacha inchi albumin exhibits considerable functional activities with notable anti-inflammatory and antioxidation properties, which could further delay skin aging. However, its underlying mechanisms of delaying skin aging have not been elucidated. The aim of present study was to investigate the anti-skin-aging effect of Sacha inchi albumin (SIA) in D-galactose induced aging mice. RESULTS SIA improved moisture content, collagen level, and the state of aged skin in rats. SIA intervention markedly increased the skin antioxidant enzymatic activities including glutathione peroxidase, and catalase but decreased the malondialdehyde content. It also regulated inflammation by reducing the levels of TNF-α and increasing the level of IL-6. In addition, SIA administration increased the expression levels of collagen I and III, upraised the expression of tissue inhibitor of metalloprotease-1, and decreased the expression of metalloproteinases. Besides, SIA could activate TGF-β/Smad pathway. Meanwhile, 16S rRNA sequencing analysis revealed that SIA treatment altered the composition of microbiota, and increased the relative abundance of Lactobacillus, but decreased the relative abundance of Alloprevotella and Helicobacter, etc. Helicobacter was positively associated with MDA content and negatively related to IL-6. CONCLUSION SIA exhibits excellent anti-skin-aging effects, which provides a new insight for the development of functional sacha inchi albumin. This article is protected by copyright. All rights reserved.