LAUSR

The roots of Dipsacus asper Wall as a commonly used traditional Chinese medicine has been used for tonifying liver and kidney and strengthening bones and muscles. However, an effective separation strategy for comprehensive and rapid separation of the main active compounds from the roots of D. asper is nonexistent. This investigation provided an effective separation method based on AB-8 macroporous resin column chromatography using different ratios of ethanol in water and two different modes of high speed counter-current chromatography with salt-containing solvent system for rapid enrichment and separation from the roots of D. asper. The macroporous resin column chromatography was performed on AB-8 resin using ethanol in water ratios of 10%, 30%, 40%, 50%, and 80% as the optimized enrichment conditions for iridoid glycosides and triterpenoid saponins with different polarities. For high speed counter-current chromatography separation, the conventional and recycling modes were combined together to develop a strategy for twelve compounds (1-12) from the enriched parts of 30%, 40%, and 80% ethanol, including six high-polarity iridoid glycosides (1-6) using inorganic salt contained solvent system and six triterpenoid saponins (7-12). Recycling high speed counter-current chromatography separation was successfully applied to separate two isomers (9 and 10) after eleven cycles. This article is protected by copyright. All rights reserved.