LAUSR

A novel ecofriendly, cost and time saving high performance thin layer chromatographic method was developed and validated for simultaneous determination of metoclopramide, ergotamine, caffeine and paracetamol in bulk and pharmaceutical formulation. The separation was carried out on silica gel plates, using ethyl acetate: ethanol: ammonia (9: 1: 0.1, by volume) as a developing system. Ultra violet detection was carried out at 272 nm. The resulting retention times were 0.15, 0.36, 0.49 and 0.74 for metoclopramide, ergotamine, caffeine, and paracetamol, respectively. The greenness profile assessment was achieved to the proposed method to evaluate its greenness characters to the environment with acceptable results. Validation parameters were checked according to International Conference of Harmonization guidelines to achieve the international requirements for quality control analysis of the proposed drugs. This article is protected by copyright. All rights reserved.