LAUSR

While Supercritical fluid chromatography was developed over fifty years ago, it is only over the past fifteen to twenty years that it has become routinely utilized. Along with the commercialization of a new generation of instruments, during the last twenty years supercritical fluid chromatography has improved performance, reliability and robustness. SFC is fully compatible with mass spectrometric techniques. This review compiles the application of supercritical fluid chromatography separations coupled to MS instrumentation for the exploration, profiling and quantitation of metabolites during the last two decades. The selection of metabolites chosen for this article have direct applications in preclinical models of disease and clinical applications as potential biomarkers of disease including lipids, steroid hormones, bile acids, polar metabolites, peptides, and proteins. This article is protected by copyright. All rights reserved.