LAUSR

This work was conducted to optimize an accelerated solvent extraction for ultra-HPLC-MS/MS analysis of blueberry phenolic compounds. The conditions for accelerated solvent extraction were verified using response surface methodology to obtain the following optimized conditions: ethanol concentration (pH = 3), 48%; temperature, 50 ℃ and static cycle times, 3. Further, ultra-HPLC with quadrupole Exactive Orbitrap/MS and ultra-HPLC with triple-quadrupole tandem mass methods for determination of the detailed phenolic composition were developed and validated. Total of 81 phenolic compounds were identified by ultra-HPLC with quadrupole Exactive Orbitrap/MS, including 23 anthocyanins, 32 flavonols, 11 proanthocyanidins, 2 other flavonoids and 13 phenolic acids. 55 of these compounds have been simultaneously quantified by ultra-HPLC with triple-quadrupole tandem mass, including 31 anthocyanins, 8 flavonols, 6 proanthocyanidins, 2 other flavonoids and 8 phenolic acids. Malvidin-dinhexoside has, for the first time, been detected in Wild. Moreover, by verifying the protection on PC12 Cells against oxidative damage, it was showed that the phenolic extracts (500 μg/mL) can improve significantly the viability (9.26%-24.78%) of hydrogen peroxide-induced PC12 Cells, activities of superoxide dismutase (34.59-37.90 U/mg) and glutathione peroxidase (6.87-14.42 mU/mg) and decrease the content of malonic dialdehyde (13.27-24.62 nmol/mg). Correlation analysis suggested that anthocyanins might contrubute most to these activities. This article is protected by copyright. All rights reserved.