LAUSR

Carthami flos, commonly known as Honghua in China, is the dried floret of safflower and widely acknowledged as a blood stasis promoting herb. The study aimed at investigating the relationship between thrombin and carthami flos (CF) through a high-performance thrombin affinity chromatography combined with HPLC-MS/MS system (TAC-HPLC-MS/MS). Firstly, thrombin was immobilized on the glutaraldehyde-modified amino silica gel to prepare the thrombin affinity stationary phase, which was packed into a small column (1.0*2.0 mm, i.d.) for recognizing the anticoagulant active components of CF. The target component was enriched and analyzed by the HPLC-MS/MS. Finally, hydroxylsafflor yellow A (HSYA) was screened out and identified as the active component. The anticoagulant effects of HSYA were analyzed by anticoagulant experiments in vitro, and the interaction of HSYA with thrombin was investigated by molecular docking method. The results proved that HYSA (30 μg/mL, 0.05 mM) and CFE (30 μg/mL) could prolong APTT and TT by 50% and 11%, respectively. Moreover, HSYA exhibits a good hydrogen bond field and stereo field matching with thrombin. Overall, it was concluded that HSYA might exert an anticoagulation effect by interacting with thrombin and thus could be potential anticoagulant drugs for the prevention and treatment of venous thrombosis. This article is protected by copyright. All rights reserved.