LAUSR

Herein, we report the preparation and application of a new nano-structured monolithic nano-column based on modified graphene oxide using narrow fused silica capillary column (e.g. 50-μm internal-diameter). The nano-column was prepared by an in-situ polymerization using butyl methacrylate, ethylene dimethacrylate and methacryloyl graphene oxide nanoparticles. Dimethyl formamide and water were used as the porogenic solvent. After polymerization, the obtained nano-column was coated with dimethyloctadecylchlorosilane in order to enhance the hydrophobicity. Both isocratic and gradient nano-liquid chromatographic separations for small molecules (e.g. alkylbenzenes) and macromolecules (e.g. intact proteins) were performed. Theoretical plates number up to 3600 plates/m in isocratic mode for propylbenzene were achieved. It was demonstrated that the feasibility of graphene oxide modified monolithic nano-column for high efficiency and high throughput nanoscale proteomics analysis. The high resolving power of monolithic nano-column yielded sensitive protein separation with narrower peak width while a high-resolution analysis of peptides from trypsin-digested cytochrome C could be obtained. Graphene oxide based monolithic nano-columns are promising and can allow to powerful tools for trace proteom sample analysis. This article is protected by copyright. All rights reserved.