LAUSR

Langdu known as a Traditional Chinese Medicine was identified as the roots of species of Euphorbia ebracteolata Hayata and Euphorbia fischeriana Steud, displaying anti-tuberculosis activity. In order to clarify the potent quality markers of Langdu, this research firstly developed a fast and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry method for the quantification of thirteen diterpenoids existed in Langdu. The developed method was further applied in the analyses of 12 authentic E. ebracteolata and E. fischeriana samples collected in the North and Southeast of China. Then, the anti-tuberculosis evaluation of 12 batches of Langdu samples was performed in vitro. Finally, Partial least squares discrimination analysis was used in the discrimination of E. ebracteolata and E. fischeriana from different origins and processing methods. Jolkinolide A (1), jolkinolide E (3), yuexiandajisu D (6), Ebractenone A (11) were identified as the key, potent diterpenoids for quality control of E. ebracteolata Hayata and E. fischeriana Steud. The present study established a qualitative chemical analysis method for Langdu (E. ebracteolata and E. fischeriana), and suggested the key bioactive components which will improve qualitative control methodology for this important medicine. This article is protected by copyright. All rights reserved.