LAUSR

Traditional Chinese medicine made from the rhizome of Atractylodes lancea (Thunb.) DC. (Compositae), known as Cangzhu. In this study, gas chromatography-mass spectrometry was used to identify and quantify the volatile oils of different organs of A. lancea from four regions of China: Jiangsu, Anhui, Henan, and Hubei provinces. The volatile oils of A. lancea were qualitatively and quantitatively characterized using GC-MS combined with laser microdissection. The results identified 21 components in A. lancea, the majority of the components were found in the rhizomes, followed by the fibrous roots, flowers, leaves, and stems. According to the contents of volatile oils in A. lancea, it was divided into Dabieshan-type (mainly includes hinesol and β-eudesmol) and Maoshan-type (mainly includes atractylon and atractylodin), the ratios of hinesol:β-eudesmol:atractylon:atractylodin were 17.06:4.55:0:1, 12.66:11.71:0.99:1, 7.43:6.23:0:1 and 0.13:0.16:1.52:1 in A. lancea from AH, HN, HB and JS, respectively. Tissue-specific study indicated that Dabieshan-type mainly includes elemol, hinesol, β-eudesmol in the periderm and secretory cavities of A. lancea, whereas Maoshan-type mainly including atractylon, atractylodin, little hinesol and β-eudesmol in the secretory cavities. Conversely, no volatile oils were detected in the cortex, phloem, xylem, vascular ray, or pith. This study provides a foundation for further evaluation and utilization of A. lancea. This article is protected by copyright. All rights reserved.