LAUSR

A novel thiolysis-HPLC method for quantitative determination of total proanthocyanidins and mean degree of polymerization in grape seeds has been developed. Following thiolysis with formic acid and benzyl mercaptan, reaction products were separated and purified. Three proanthocyanidin monomers and three derivatives were obtained and their structures were identified by LC-MS, fourier transform infrared spectroscopy, and NMR. A decomposition model of the thiolysis products and a correction formula for proanthocyanidins concentration were established. This thiolysis-HPLC method displayed good calibration linearity (R2 > 0.999 over the concentration range 0.01 to 10 mg/mL), and excellent accuracy (recoveries of 97.9-99.6%) and precision (repeatability relative standard deviations of 0.45-0.75%). This method is suitable for the quantitative analysis of proanthocyanidins in grape seed products. This article is protected by copyright. All rights reserved.