LAUSR

The illegal use of clenbuterol seriously endangers food safety and human health. Accurate monitoring the illegal use of clenbuterol in livestock can efficiently prevent the clenbuterol residue pork products from entering the consumer market. Thus, in this study, a simple, rapid and sensitive method for the determination of clenbuterol in swine urine was developed using electromembrane extraction combined with liquid chromatography-tandem mass spectrometry. It should be noted that the electromembrane extraction method presented many advantages of simple operation, fast mass transfer rate, good sample clean-up capability and less organic solvent consumption. The effect of important factors on the extraction efficiency of clenbuterol was investigated. Under the optimal conditions, good linearity was achieved for clenbuterol over the range of 1-1000 ng/mL (R2 = 0.9996). The recoveries of clenbuterol in swine urine at three spiked levels ranged from 83.7 to 110.0% with relative standard deviation values lower than 9.7% (n = 4). Limits of detection and quantification for clenbuterol were 0.07 and 0.25 ng/mL, respectively. These results suggested that the proposed method has great potential on the extraction and determination of trace analyte in complex sample matrix for monitoring the illegal use in livestock. This article is protected by copyright. All rights reserved.