LAUSR

Due to green and environment-friendly characteristics, the ultra-high performance supercritical fluid chromatography has been widely used in analytical fields in recent years, but until to now few reports are available for monosaccharide compositional analysis of macromolecule polysaccharides. In this study, an ultra-high performance supercritical fluid chromatography technology with an unusual binary modifier is used to determine monosaccharide compositions of natural polysaccharides. Each carbohydrate herein is simultaneously labeled as 1-pheny-3-methyl-5-pyrazolone and acetyl-derivative via pre-column derivatizations for aiming to increase ultraviolet absorption sensitivity and decrease water solubility. 10 common monosaccharides are fully separated and detected on ultra-high performance supercritical fluid chromatography combined with photo-diode array detector by a systematic optimization of multiple relevant parameters, e.g., column stationary phases, organic modifiers, additives, flow rates and so on. Compared with carbon dioxide as mobile phase, the addition of binary modifier increases the resolution of analytes. Additionally, this method has the advantages of small consumption for organic solvent, safety and environmental-friendly. It has been successfully applied for full monosaccharide compositional analysis of heteropolysaccharides from Schisandra chinensis fruits. To sum up, a new alternative approach is provided for monosaccharide compositional analysis of natural polysaccharides. This article is protected by copyright. All rights reserved.