LAUSR

Decellularized extracellular matrices are being extensively used for tissue engineering purposes and detergents are predominantly used for this. We have developed a facile detergent-free decellularization method for dermal matrix and compared it with the most used detergent based decellularization methods. An optimized, single-step, cost-effective Hypo/Hyper (H/H) molar NaCl solutions-based method was employed to decellularize Goat skin that resulted in much higher yield than other methods. The ECM composition, mechanical property, and cytocompatibility were evaluated and compared with other decellularization methods. Furthermore, our H/H treated decellularized dermal ECM (ddECM) exhibited a residual DNA content of < 50 ng/mg of dry tissue. Moreover, 85.64 ± 3.01% of glycosaminoglycans (GAGs) and 65.53 ± 2.9% collagen were retained compared to the native tissue, which is higher than the ddECMs prepared by other methods. The cellular response was superior in ddECM (H/H) than other ddECMs prepared by detergent-based methods. Additionally, a bioink was formulated with the ddECM (H/H), showing good shear thinning and shear recovery properties. Process optimization in terms of print speed, flow rate, and viscosity was done to obtain a bioprinting window for ddECM bioink. The printed constructs with optimized parameters had adequate mechanical and cell adhesive properties and excellent isotropic cellular alignment. This article is protected by copyright. All rights reserved.