LAUSR

Body-fluids, cells and tissues contain a wide variety of metabolites that consist of a mixture of various low-molecular-weight compounds that includes amino acids/peptides, lipids, nucleic acids, and organic acids, which makes the comprehensive analysis more difficult. Quantitative nuclear magnetic resonance spectroscopy (NMR) is a well-established analytical technique for analyzing the metabolic profiles of body fluids, cells, and tissues. It enables fast and comprehensive detection, characterization, high level of experimental reproducibility, minimal sample preparation, and quantification of various endogenous metabolites. In latest times, NMR-based metabolomics has been appreciably utilized in diverse branches of medicine, including microbiology, toxicology, pathophysiology, pharmacology, nutritional intervention, and disease diagnosis/prognosis. In this review, the utility of NMR-based metabolomics in clinical studies are discussed. The significance of in vitro NMR-based metabolomics as an effective device for detecting metabolites and their variations in different diseases are discussed together with a possibility of identifying specific biomarkers that can contribute to the early detection and diagnosis of the disease.