Background: Mass spectrometry (MS)‐based proteomic analysis of posttranslational modifications (PTMs) usually requires the pre‐enrichment of modified proteins or peptides. However, recent ultra‐deep whole proteome profiling generates millions of spectra in… Click to show full abstract
Background: Mass spectrometry (MS)‐based proteomic analysis of posttranslational modifications (PTMs) usually requires the pre‐enrichment of modified proteins or peptides. However, recent ultra‐deep whole proteome profiling generates millions of spectra in a single experiment, leaving many unassigned spectra, some of which may be derived from PTM peptides.
               
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