LAUSR

Mass spectrometry has long been utilized to characterize a variety of biomolecules such as proteins, metabolites, and lipids. Most MS‐based omics studies rely on bulk analysis; however, bulk approaches often overlook low‐abundance molecules that may exert critical biological effects. Recently, multi‐omics analyses have been driving an explosion of knowledge about how biomolecules interact within biological systems. In particular, spatial multi‐omics has emerged as a groundbreaking approach for implementing multi‐omic and multi‐modal analyses. Broadly defined, spatial omics has the ability to analyze biomolecules within their native spatial contexts, offering transformative insights. This review focuses on mass spectrometry‐based spatial omics, specifically matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI‐MSI). We will explore how MALDI‐MSI, in combination with laser capture microdissection (LCM) and traditional liquid chromatography‐mass spectrometry (LC‐MS) workflow, is advancing spatially resolved multi‐omics research.