LAUSR

Radiation‐induced manifestations like free radical burst, oxidative damage and apoptosis leading to cell death. In present study, N‐acetyl tryptophan glucopyranoside (NATG) was assessed for its immune‐radioprotective activities using J774A.1 cells. Clonogenic cell survival, cell cycle progression and cytokines i.e. IFN‐γ, TNF‐α, IL‐2, IL‐10, IL‐12, IL‐13 and IL‐17A expression were evaluated in irradiated and NATG pretreated cells using clonogenic formation ability, flow cytometry and ELISA assay. Results indicated that 0.25μg/ml NATG exhibited maximum radioprotection against gamma‐radiation (2Gy) without intervening in cell cycle progression. NATG pretreated (−2 h) plus irradiated cells showed significant elevation in IFN‐γ (∼38.2%), IL‐17A (∼53.7%) and IL‐12 (∼58.8%) expression as compared to only irradiated cells. Conversely, significant decrease in TNF‐α (∼21.6%), IL‐10 (∼31.2%), IL‐2 (∼23.7%) and IL‐13 expression (∼17.8%) were observed in NATG pretreated plus irradiated cells as compared to irradiated cells. Conclusively, NATG pretreatment to irradiated J774A.1 cells, stimulate Th1 while diminish Th2 cytokines that contributes to radioprotection.