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The isolation and culture of single floating myofibers with their adjacent muscle stem cells allow the analysis and comparison of muscle stem cells from aged and young mice. This method… Click to show full abstract
The isolation and culture of single floating myofibers with their adjacent muscle stem cells allow the analysis and comparison of muscle stem cells from aged and young mice. This method has the advantage that muscle stem cells are cultured on the myofiber, thereby culturing them in conditions as close to their endogenous niche as possible. Here we describe the isolation, culture, transfection with siRNA, and subsequent immunostaining for muscle stem cells on their adjacent myofibers from aged and young mice.
Keywords:
muscle;
stem cells;
muscle stem;
isolation culture
Journal Title: Methods in molecular biology
Year Published: 2019
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2019
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!