Photo from wikipedia
The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from… Click to show full abstract
The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells using CRISPR/Cas9. Editing frequencies of up to 90% and integration rates greater than 60% can be achieved with this method.
Keywords:
primary human;
crispr cas9;
engineering primary;
genome engineering
Journal Title: Methods in molecular biology
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!
Journal Title: Methods in molecular biology
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!