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Detection of Influenza A Antibodies in Avian Samples by ELISA.

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ELISA assays are a fast and relatively inexpensive way to screen sera for antibodies to avian influenza virus. Commercial ELISA kits are available, and although they are more expensive than… Click to show full abstract

ELISA assays are a fast and relatively inexpensive way to screen sera for antibodies to avian influenza virus. Commercial ELISA kits are available, and although they are more expensive than in-house tests, they provide a ready-to-use assay with good quality control. Numerous sample types can be processed for ELISA: serum, plasma, egg yolk, or blood collected on filter paper. High-quality samples are critical to accurate results. The basics of AIV antibody ELISA, sample processing, result interpretation, and troubleshooting are discussed.

Keywords: avian samples; samples elisa; influenza; influenza antibodies; antibodies avian; detection influenza

Journal Title: Methods in molecular biology
Year Published: 2020

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