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Malectin is a membrane-anchored endoplasmic reticulum (ER)-resident lectin, which is first identified in Xenopus laevis in 2008 and highly conserved among animals. Malectin plays an important role in the quality… Click to show full abstract
Malectin is a membrane-anchored endoplasmic reticulum (ER)-resident lectin, which is first identified in Xenopus laevis in 2008 and highly conserved among animals. Malectin plays an important role in the quality control of glycoprotein in the ER through recognizing the Glc2Man9GlcNAc2 (G2M9) oligosaccharide chain on the newly synthesized glycoproteins. In this chapter, we will describe the preparation of recombinant soluble malectin and its tetramer, which might be developed as useful tools for detection of GlcĪ±1-3Glc containing glycans on the cell surface.
Journal Title: Methods in molecular biology
Year Published: 2020
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