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Laser microdissection (LMD) technology has been widely applied to plant tissues, offering novel information on the role of different cell-type populations during plant-microbe interactions. In this chapter, protocols to apply… Click to show full abstract
Laser microdissection (LMD) technology has been widely applied to plant tissues, offering novel information on the role of different cell-type populations during plant-microbe interactions. In this chapter, protocols to apply the LMD approach to study plant and fungal transcript profiles in different cell-type populations from arbuscular mycorrhizal (AM) roots are described in detail, starting from the biological material preparation to gene expression analyses by RT-PCR and RT-qPCR.
Keywords:
plant fungal;
plant;
gene expression;
study;
laser microdissection
Journal Title: Methods in molecular biology
Year Published: 2020
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!