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Localization, Extraction, and Quantification of Plant and Algal Arabinogalactan Proteins.

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Arabinogalactan proteins are a diverse group of cell wall-associated proteoglycans. While structural and molecular genetic analyses have contributed to the emerging improved understanding of the wide-range of biological processes in… Click to show full abstract

Arabinogalactan proteins are a diverse group of cell wall-associated proteoglycans. While structural and molecular genetic analyses have contributed to the emerging improved understanding of the wide-range of biological processes in which AGPs are implicated; the ability to detect, localize, and quantify them is fundamentally important. This chapter describes three methods: histological staining, radial gel diffusion, and colorimetric quantification, each of which utilize the ability of Yariv reagent to bind to AGPs.

Keywords: arabinogalactan proteins; localization extraction; plant algal; quantification; extraction quantification; quantification plant

Journal Title: Methods in molecular biology
Year Published: 2020

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