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Overexpression of RFP-tagged proteins in growing tobacco pollen tubes together with the genetically encoded Ca2+ sensor YC3.6 allows to analyze localization and dynamics of the protein of interest, as well… Click to show full abstract
Overexpression of RFP-tagged proteins in growing tobacco pollen tubes together with the genetically encoded Ca2+ sensor YC3.6 allows to analyze localization and dynamics of the protein of interest, as well as the impact of its overexpression on Ca2+ dynamics and pollen tube growth. Here, we describe a step-by-step instruction for transient transformation of N. tabacum pollen and subsequent in vitro germination and Ca2+ imaging.
Keywords:
overexpression;
tobacco pollen;
ca2;
pollen tubes;
overexpression ca2
Journal Title: Methods in molecular biology
Year Published: 2020
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!