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Kupffer cells and hepatocytes maintain liver homeostasis. These cells could be separated based on their size and weight, by centrifugation using a density gradient after a liver perfusion. Here, we… Click to show full abstract
Kupffer cells and hepatocytes maintain liver homeostasis. These cells could be separated based on their size and weight, by centrifugation using a density gradient after a liver perfusion. Here, we describe a methodology to isolate both Kupffer cells and hepatocytes from a single mouse, which provides the unique advantage of studying these two cell types from the same liver.
Keywords:
single mouse;
kupffer;
liver;
centrifugation;
gradient
Journal Title: Methods in molecular biology
Year Published: 2020
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!