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Virus-induced gene silencing (VIGS) has been successfully applied for functional analysis of genes in many plant species. Many protocols have been established but mainly for gene expression study in vegetative… Click to show full abstract
Virus-induced gene silencing (VIGS) has been successfully applied for functional analysis of genes in many plant species. Many protocols have been established but mainly for gene expression study in vegetative tissue. Here, we present the critical steps of an optimized procedure of VIGS in flax (Linum usitatissimum L.) seed coat using the transparent testa 2 gene as a selectable marker. The present protocol may serve as an effective tool for functional characterization of genes involved in seed coat formation and/or biological functions.
Journal Title: Methods in molecular biology
Year Published: 2020
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