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The analysis of protein enrichment in the detergent-resistant membranes (DRMs) isolated from immune cells enables us to analyze a link between the membrane lipid dynamics and cell activation. Here, we… Click to show full abstract
The analysis of protein enrichment in the detergent-resistant membranes (DRMs) isolated from immune cells enables us to analyze a link between the membrane lipid dynamics and cell activation. Here, we describe the fractionation of detergent-resistant membranes and the correlative analysis of the enrichment of T cell receptor (TCR) and ω-azido-modified synthetic ceramide in those fractions upon TCR stimulation.
Keywords:
functionalized sphingolipid;
resistant membranes;
detection functionalized;
immune cells;
detergent resistant
Journal Title: Methods in molecular biology
Year Published: 2021
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2021
Link to full text (if available)
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recommendations!