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The success of any oligonucleotide-based experiment strongly depends on the accurate design of the components. Oli2go is a user-friendly web tool that provides efficient multiplex oligonucleotide design including specificity and… Click to show full abstract
The success of any oligonucleotide-based experiment strongly depends on the accurate design of the components. Oli2go is a user-friendly web tool that provides efficient multiplex oligonucleotide design including specificity and primer dimer checks. Its fully automated workflow involves important design steps that use specific parameters to produce high-quality oligonucleotides. This chapter describes how these steps are computationally implemented by oli2go.
Keywords:
computational design;
design multiplex;
multiplex oligonucleotide;
based assays;
oligonucleotide based;
design
Journal Title: Methods in molecular biology
Year Published: 2021
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2021
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!