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Automation of Continuous-Rotation Data Collection for MicroED.

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Automated coordination of microscope and camera functions for MicroED data collection simplifies the procedure for robust dataset acquisition and enables unattended sequential collection of many crystal targets. This chapter discusses… Click to show full abstract

Automated coordination of microscope and camera functions for MicroED data collection simplifies the procedure for robust dataset acquisition and enables unattended sequential collection of many crystal targets. This chapter discusses the prerequisites for an algorithm of data collection automation for continuous-rotation MicroED and presents a practical protocol for achieving this goal using the popular TEM control software program SerialEM.

Keywords: data collection; collection; automation continuous; continuous rotation

Journal Title: Methods in molecular biology
Year Published: 2021

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