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C3dg Quantification by PEG Precipitation and or TRIFMA.

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Detection of complement activation products can be carried out in a number of ways, and different methods are used in different laboratories. No international standard for measuring complement activation in… Click to show full abstract

Detection of complement activation products can be carried out in a number of ways, and different methods are used in different laboratories. No international standard for measuring complement activation in the clinical setting has been agreed upon.Here we describe a modified assay for measuring C3dg. The assay is simple, inexpensive and stable. The estimation of C3dg directly reflects complement turnover independently of activation pathway.

Keywords: c3dg quantification; peg precipitation; precipitation trifma; biology; quantification peg

Journal Title: Methods in molecular biology
Year Published: 2021

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