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Open the Pores: Electroporation for the Transformation of Trichoderma reesei.

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During the electroporation of T. reesei, linearized exogenous DNA is absorbed into swollen conidia by an electrical impulse. The advantage of this method is that it is less time-consuming, less… Click to show full abstract

During the electroporation of T. reesei, linearized exogenous DNA is absorbed into swollen conidia by an electrical impulse. The advantage of this method is that it is less time-consuming, less expensive, and easier to perform than the classical protoplast transformation while at the same time having a comparable efficiency.

Keywords: electroporation transformation; electroporation; open pores; reesei; pores electroporation

Journal Title: Methods in molecular biology
Year Published: 2021

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