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Targeted chromatin capture (T2C) is a 3C-based method and is used to study the 3D chromatin organization, interactomes and structural changes associated with gene regulation, progression through the cell cycle,… Click to show full abstract
Targeted chromatin capture (T2C) is a 3C-based method and is used to study the 3D chromatin organization, interactomes and structural changes associated with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinformatics tools in detail.
Keywords:
chromatin capture;
low input;
low t2c;
targeted chromatin
Journal Title: Methods in molecular biology
Year Published: 2021
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Journal Title: Methods in molecular biology
Year Published: 2021
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!