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Fmoc Solid Phase Peptide Synthesis of Oxytocin and Analogues.

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Solid phase peptide synthesis is the most commonly used method for the production of peptides. In this chapter, we outline the standard operating procedures used in our laboratory to efficiently access… Click to show full abstract

Solid phase peptide synthesis is the most commonly used method for the production of peptides. In this chapter, we outline the standard operating procedures used in our laboratory to efficiently access oxytocin-like peptides. This includes detailed descriptions of equipment setup, reagent selection, peptide assembly on solid support, peptide side chain deprotection and cleavage from the solid support, oxidative folding, purification, and analysis.

Keywords: phase peptide; fmoc solid; solid phase; peptide synthesis

Journal Title: Methods in molecular biology
Year Published: 2022

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