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Mycolactones are a family of polyketide synthase products made by the human pathogen Mycobacterium ulcerans that were recently identified as novel inhibitors of the host membrane translocation complex (Sec61). Here,… Click to show full abstract
Mycolactones are a family of polyketide synthase products made by the human pathogen Mycobacterium ulcerans that were recently identified as novel inhibitors of the host membrane translocation complex (Sec61). Here, we provide protocols for the purification of mycolactones from bacterial cultures, and for their quantitative assessment in biological samples.
Keywords:
cultures hplc;
mycolactone purification;
purification ulcerans;
biological samples;
ulcerans cultures;
purification
Journal Title: Methods in molecular biology
Year Published: 2022
Link to full text (if available)
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Journal Title: Methods in molecular biology
Year Published: 2022
Link to full text (if available)
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recommendations!