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High Spatial Resolution Luciferase Imaging of the Arabidopsis thaliana Circadian Clock.

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The A. thaliana circadian clock is an example of a gene network that generates rich temporal and spatial dynamics. Bioluminescent imaging has proven a powerful method to help dissect the… Click to show full abstract

The A. thaliana circadian clock is an example of a gene network that generates rich temporal and spatial dynamics. Bioluminescent imaging has proven a powerful method to help dissect the genetic mechanisms that generate oscillations of gene expression over the course of the day. However, its use for the study of spatial regulation is often limited by resolution. Here, we describe a modified luciferase imaging method for the study of the Arabidopsis circadian clock across the plant at sub-tissue-level resolution.

Keywords: resolution; thaliana circadian; luciferase imaging; circadian clock; clock

Journal Title: Methods in molecular biology
Year Published: 2022

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