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Stress granule (SG)-based RNA interference (RNAi) screening is a powerful method to discover factors that control protein synthesis and aggregation, as well as regulators of SG assembly and disassembly. Here,… Click to show full abstract
Stress granule (SG)-based RNA interference (RNAi) screening is a powerful method to discover factors that control protein synthesis and aggregation, as well as regulators of SG assembly and disassembly. Here, we describe how to set up and optimize a large-scale siRNA screen, and give a detailed outline for the automated quantification of SGs as a visual readout. Hit evaluation via calculated Z scores provides a list of candidates for further in-depth studies.
Keywords:
image based;
stress;
stress granule;
based screening;
screening stress
Journal Title: Methods in molecular biology
Year Published: 2022
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Journal Title: Methods in molecular biology
Year Published: 2022
Link to full text (if available)
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recommendations!